PROTEOLYTIC ACTIVITY CHANGES OF HOMOGENATES OF CHLORELLA VULGARIS CELLS AND CULTURE FUNCTION-METABOLIC REORGANIZATIONS AT THE GROWTH IN THE MnCl2 PRESENCE
Keywords:
chlorella culture, proteolysis, manganese (II) chloride, protein cleavageAbstract
The changes of proteolytic activity at рН 3.0, 7.4 and 9.0 of Chlorella vulgaris cell homogenates were studied by gelatin and fibrinogen lysis method in thin-layer gel at 10, 16, 22 and 40 days of culture growth when manganese chloride was added to the medium to final concentration of
0.025, 1.0 and 10.0 mg/l and were compared with shifts of intracellular protein level. The presence of cell functional metabolic reorganizations was confirmed. It has been shown that the cell proteolytic activity and the level of intracellular protein in a number of cases change in antibathic manner. It has been suggested that these proteolytic activity shifts possess regulatory character.
References
1. Suman, G. Single cell protein production: A review / G. Suman, M. Nupur, S. Anuradha, B. Pradeep // Int. J. Curr. Microbiol. App. Sci. – 2015. – Vol. 4. – P. 251–262.
2. Ильючик, И.А. Рост культуры хлореллы (Chlorella vulgaris) и накопление белка при добавлении MnCl2 в питательную среду / И.А. Ильючик, В.Н. Никандров // Веснік Палескага дзяржаўнага універсітэта. Серыя прыродазнаўчых навук. – 2018. – № 1. – С. 53-64.
3. Zablocka-Slowinska, K. The role of manganese in etiopathogenesis and prevention of selected diseases / K. Zablocka-Slowinska, H. Grajeta // Postepy
Hig. Med. Dosw. (Online). – 2012. – Vol. 66. – P. 549–553.
4. Lin, Y.T. Manganous ion supplementation accelerates wild type development, enhances stress resistance, and rescues the life span of a short-lived Caenorhabditis elegans mutant // Y.T. Lin, H. Hoang, S.I. Hsieh, N. Rangel, A.L. Foster, J.N. Sampayo, G.J. Lithgow, С. Srinivasan // Free Radic. Biol. Med. – 2006. – Vol. 40, No. 7. – Р. 1185–1193.
5. Макарова, Е.И. Прикладные аспекты применения микроводорослей – обитателей водных экосистем / Е.И. Макарова, И.П. Отурина, А.И. Сидякин // Экосистемы, их оптимизация и охрана. – 2009. – Вып. 20. – С. 120–133.
6. Музафаров, А.М. Культивирование и применение микроводорослей / А.М. Музафаров, Т.Т. Таубаев. – Ташкент: Фан УзССР. – 1984. – 136 с.
7. Bleakley, S. Algal рroteins: еxtraction, аpplication, and сhallenges сoncerning рroduction / S. Bleakley, M. Hayes // Foods. – 2017. – Vol. 6, No. 5. – Р. 33.
8. Никандров, В.Н. Методы исследования протеолиза. Глава 5 / В.Н. Никандров, Н.С. Пыжова // Современные проблемы биохимии. Методы исследований. – Минск: Выш. шк. – 2013. – С. 132–157.
9. Ильючик, И.А. О роли протеолиза в функционально-метаболических перестройках клеток Chlorella vulgaris при действии MnCl2 / И.А. Ильючик, В.Н. Никандров // Регуляция роста, развития и продуктивности растений: материалы IX междунар. науч. конф., Минск, 24 -26 окт. 2018 г. /
Институт экспериментальной ботаники им. В.Ф. Купревича НАН Беларуси. – Минск: Колорград, 2018. – С.50.
References
1. Suman G., Nupur M, Anuradha S., Pradeep B. Single cell protein production: A review . Int. J. Curr. Microbiol. App. Sci., 2015, Vol. 4, pp. 251-262.
2. Ilyuchik I.A., Nikandrov V.N. Rost kulturyi hlorellyi (Chlorella vulgaris) i nakoplenie belka pri dobavlenii MnCl 2 v pitatelnuyu sredu [Chlorella vulgaris culture growth and protein accumulation at MnCl2 addition in nutrient medium]. Vesnik Paleskaga dzyarzhaўnaga unIversIteta. Seryiya pryirodaznaўchyih navuk [Bulletin of Polessky State University. Series in Natural Sciences], 2018, no. 1, pp. 53-64 (In Russian).
3. Zablocka-Slowinska K., Grajeta H. The role of manganese in etiopathogenesis and prevention of selected diseases. Postepy Hig. Med. Dosw, 2012, vol. 66, pp. 549-553. (In Russian).
4. Lin Y.T., Hoang Н., Hsieh S.I., Rangel N., Foster A.L., Sampayo J.N., Lithgow G.J., Srinivasan С. Manganous ion supplementation accelerates wild type development, enhances stress resistance, and rescues the life span of a short–lived Caenorhabditis elegans mutant. Free Radic. Biol. Med. 2006. Vol. 40, no. 7б рр. 1185-1193.
5. Makarova E.I., Oturina I.P., Sidyakin A.I. Prikladnye aspekty primeneniya mikrovodoroslej – obitatelej vodnyh [Applied aspects of the use of microalgae -the inhabitants of water]. Ehkosistemy, ih optimizaciya i ohrana [Ecosystems, their optimization and protection], 2009, iss. 20, pp. 120-133. (In Russian).
6. Muzafarov A.M., Taubaev T.T. Kul'tivirovanie i primenenie mikrovodoroslej [Cultivation and use of microalgae]. Tashkent, 1984. 136 p. (In Russian).
7. Bleakley S., Hayes M. Algal рroteins: еxtraction, аpplication, and сhallenges сoncerning рroduction. Foods, 2017, vol. 6, no. 5, p. 33.
8. Nikandrov V.N., Pyizhova N.S. Metodyi issledovaniya proteoliza. Glava 5. [Methods for the study of proteolysis. Chapter 5]. Sovremennyie problemyi biohimii. Metodyi issledovaniy [Modern problems of biochemistry. Research methods]. Minsk: Visheyshaya shkola, 2013, рр. 132-157. (In Russian).
9. Ilyuchik I.A., Nikandrov V.N. O roli proteoliza v funkcional'no-metabolicheskih perestrojkah kletok Chlorella vulgaris pri dejstvii MnCl2 [On the role of proteolysis in functional metabolic rearrangements of Chlorella vulgaris cells under the action of MnCl2]. Regulyaciya rosta, razvitiya i produktivnosti rastenij: materialy IX mezhdunarodnoj nauchoj konferencii.[Regulation of growth, development and productivity of plants]. Institute of Experimental Botany.
V.F. Kuprevich NAS of Belarus. Minsk: Kolorgrad, 2018, p. 50. (In Russian).
2. Ильючик, И.А. Рост культуры хлореллы (Chlorella vulgaris) и накопление белка при добавлении MnCl2 в питательную среду / И.А. Ильючик, В.Н. Никандров // Веснік Палескага дзяржаўнага універсітэта. Серыя прыродазнаўчых навук. – 2018. – № 1. – С. 53-64.
3. Zablocka-Slowinska, K. The role of manganese in etiopathogenesis and prevention of selected diseases / K. Zablocka-Slowinska, H. Grajeta // Postepy
Hig. Med. Dosw. (Online). – 2012. – Vol. 66. – P. 549–553.
4. Lin, Y.T. Manganous ion supplementation accelerates wild type development, enhances stress resistance, and rescues the life span of a short-lived Caenorhabditis elegans mutant // Y.T. Lin, H. Hoang, S.I. Hsieh, N. Rangel, A.L. Foster, J.N. Sampayo, G.J. Lithgow, С. Srinivasan // Free Radic. Biol. Med. – 2006. – Vol. 40, No. 7. – Р. 1185–1193.
5. Макарова, Е.И. Прикладные аспекты применения микроводорослей – обитателей водных экосистем / Е.И. Макарова, И.П. Отурина, А.И. Сидякин // Экосистемы, их оптимизация и охрана. – 2009. – Вып. 20. – С. 120–133.
6. Музафаров, А.М. Культивирование и применение микроводорослей / А.М. Музафаров, Т.Т. Таубаев. – Ташкент: Фан УзССР. – 1984. – 136 с.
7. Bleakley, S. Algal рroteins: еxtraction, аpplication, and сhallenges сoncerning рroduction / S. Bleakley, M. Hayes // Foods. – 2017. – Vol. 6, No. 5. – Р. 33.
8. Никандров, В.Н. Методы исследования протеолиза. Глава 5 / В.Н. Никандров, Н.С. Пыжова // Современные проблемы биохимии. Методы исследований. – Минск: Выш. шк. – 2013. – С. 132–157.
9. Ильючик, И.А. О роли протеолиза в функционально-метаболических перестройках клеток Chlorella vulgaris при действии MnCl2 / И.А. Ильючик, В.Н. Никандров // Регуляция роста, развития и продуктивности растений: материалы IX междунар. науч. конф., Минск, 24 -26 окт. 2018 г. /
Институт экспериментальной ботаники им. В.Ф. Купревича НАН Беларуси. – Минск: Колорград, 2018. – С.50.
References
1. Suman G., Nupur M, Anuradha S., Pradeep B. Single cell protein production: A review . Int. J. Curr. Microbiol. App. Sci., 2015, Vol. 4, pp. 251-262.
2. Ilyuchik I.A., Nikandrov V.N. Rost kulturyi hlorellyi (Chlorella vulgaris) i nakoplenie belka pri dobavlenii MnCl 2 v pitatelnuyu sredu [Chlorella vulgaris culture growth and protein accumulation at MnCl2 addition in nutrient medium]. Vesnik Paleskaga dzyarzhaўnaga unIversIteta. Seryiya pryirodaznaўchyih navuk [Bulletin of Polessky State University. Series in Natural Sciences], 2018, no. 1, pp. 53-64 (In Russian).
3. Zablocka-Slowinska K., Grajeta H. The role of manganese in etiopathogenesis and prevention of selected diseases. Postepy Hig. Med. Dosw, 2012, vol. 66, pp. 549-553. (In Russian).
4. Lin Y.T., Hoang Н., Hsieh S.I., Rangel N., Foster A.L., Sampayo J.N., Lithgow G.J., Srinivasan С. Manganous ion supplementation accelerates wild type development, enhances stress resistance, and rescues the life span of a short–lived Caenorhabditis elegans mutant. Free Radic. Biol. Med. 2006. Vol. 40, no. 7б рр. 1185-1193.
5. Makarova E.I., Oturina I.P., Sidyakin A.I. Prikladnye aspekty primeneniya mikrovodoroslej – obitatelej vodnyh [Applied aspects of the use of microalgae -the inhabitants of water]. Ehkosistemy, ih optimizaciya i ohrana [Ecosystems, their optimization and protection], 2009, iss. 20, pp. 120-133. (In Russian).
6. Muzafarov A.M., Taubaev T.T. Kul'tivirovanie i primenenie mikrovodoroslej [Cultivation and use of microalgae]. Tashkent, 1984. 136 p. (In Russian).
7. Bleakley S., Hayes M. Algal рroteins: еxtraction, аpplication, and сhallenges сoncerning рroduction. Foods, 2017, vol. 6, no. 5, p. 33.
8. Nikandrov V.N., Pyizhova N.S. Metodyi issledovaniya proteoliza. Glava 5. [Methods for the study of proteolysis. Chapter 5]. Sovremennyie problemyi biohimii. Metodyi issledovaniy [Modern problems of biochemistry. Research methods]. Minsk: Visheyshaya shkola, 2013, рр. 132-157. (In Russian).
9. Ilyuchik I.A., Nikandrov V.N. O roli proteoliza v funkcional'no-metabolicheskih perestrojkah kletok Chlorella vulgaris pri dejstvii MnCl2 [On the role of proteolysis in functional metabolic rearrangements of Chlorella vulgaris cells under the action of MnCl2]. Regulyaciya rosta, razvitiya i produktivnosti rastenij: materialy IX mezhdunarodnoj nauchoj konferencii.[Regulation of growth, development and productivity of plants]. Institute of Experimental Botany.
V.F. Kuprevich NAS of Belarus. Minsk: Kolorgrad, 2018, p. 50. (In Russian).
