SEASONAL CHANGES IN NITROGEN-CONTAINING COMPOUNDS OF LYMNAEA STAGNALIS AND PLANORBARIUS CORNEUS TISSUES
Keywords:
pulmonary mollusks, Lymnaea stagnalis, Planorbarius corneus, total protein, RNA, DNA, uric acid, ureaAbstract
Pulmonary freshwater mollusks pond snail and horn coil belong to species with different types of oxygen transport (copper-containing hemocyanin and iron-containing hemoglobin) and are universal test organisms for assessing the state of aquatic ecosystems of Belarus and bioecological studies. The content of urea, uric acid, DNA, RNA and total protein in hepatopancreas and hemolymph is influenced
by the season, habitat and type of oxygen transport, this allows you to form ideas about the features of nitrogen metabolism in mollusks living in natural waters, which are influenced by various anthropogenic environmental factors.
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2. Bouétard, A. Pyrosequencing-based transcriptomic resources in the pond snail Lymnaea stagnalis, with a focus on genes involved in molecular response to diquat-induced stress/ A. Bouétard [and etc.] // Ecotoxicology. – 2012. – Vol. 21 №8. – Р. 2222-2234.
3. Coutellec, M.A. Population genetics of Lymnaea stagnalis experimentally exposed to cocktails of pesticides / M.A. Coutellec, A.L. Besnard, T. Caquet // Ecotoxicology. – 2013. –Vol. 22 №5. – Р. 879-888.
4. Биохимия филогенеза и онтогенеза: учебное пособие / А.А. Чиркин [и др.]; под общ. Ред А.А. Чиркина. – Минск: Новое знание, Москва: ИНФРА-М, 2012. – 288 с.
5. Гемоглобины и гемоцианины беспозвоночных (Биохимические адаптации к условиям среды) / И.О. Алякринская. – М.: Наука, 1979. – 153 с.
6. Стадниченко, А.П. Сравнительная характеристика белкового спектра гемолимфы некоторых видов группы Lymnaea lamarck (Gastropoda) / А.П. Стадниченко // Вестник зоологии – 1974. – №5. – С. 33-37.
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9. Чиркин, А.А. Липидный обмен / А.А. Чиркин, Э.А. Доценко, Г.И. Юпатов. – Москва : Медицинская литература, 2003. – 122 с.
10. Lowry, О.Н. Protein measurement with Folin phenol reagent / О.Н. Lowry // Journal Biological Chemistry. – 1951. – Vol. 193, № 1. – P. 265-275.
11. Вlober, G. Distribution of radioactivity between the acid- soluble pool and pools of RNA in the nuclear, non sedimentable and ribosome fractions of rat liver after a single injection of labeled orotic acid / G. Вlober, V.R. Роttеr // Biochem. Biophys. Acta – 1968. – Vol. 166. – P. 48- 54.
12. Курамшина, Н.Г. Биохимические особенности плазмы гастропод (Mollusca: Gastropoda) в условиях различных уровней техногенной нагрузки на территории Башкортостана / Н. Г. Курамшина [и др.] // Материалы ІІІ Международной научной конференции «Биоразнообразие и роль зооценозов в природных и антропогенных экосистемах». – Д., 2005. – С. 46-47.
References
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2. Bouétard A. Pyrosequencing-based transcriptomic resources in the pond snail Lymnaea stagnalis, with a focus on genes involved in molecular response to diquat-induced stress. Ecotoxicology, 2012, Vol. 21, no. 8, pp. 2222-2234.
3. Coutellec M.A. Population genetics of Lymnaea stagnalis experimentally exposed to cocktails of pesticides. Ecotoxicology, 2013, Vol. 22, no. 5, pp. 879-888.
4. Chirkin A.A. et al. Biohimiya filogeneza i ontogeneza: uchebnoe posobie [Biochemistry of phylogenesis and ontogeny: teaching materials]. Eds. Chirkin A.A. Minsk, Novoe znanie, Moskva, INFRA-M, 2012, 288 p. (In Russian).
5. Aliakrinskaya I.О. Gemoglobini i gemotsianini bespozvonochnikh (Biokhemicheskiye adaptatsii k usloviyam sredi) [Hemoglobines and Hemocianines of
Invertebrates (Biochemical Adaptations to Environment)]. Moskva, Nauka, 1979, 153 p. (In Russian).
6. Stadnichenko A.P. Sravnitel'naya harakteristika belkovogo spektra gemolimfy nekotoryh vidov gruppy Lymnaea lamarck (Gastropoda) [Comparative characteristics of the protein spectrum of the hemolymph of some species of the group Lymnaea lamarck (Gastropoda)]. Vestnik zoologii [Vestnik of
zoology], 1974, no. 5, pp. 33-37 (In Russian).
7. Stadnichenko A.P., Ivanenko L.D., Vitkovskaya O.V., Kalinina N.N. Vliyanie trematodnoj invazii na soderzhanie gemocianina v gemolimfe prudovika
(Gastropoda :Pulmonata: Lymnaeidae) [Influence of trematode invasion on the content of hemocyanin in the pond gel hemolymph (Gastropoda: Pulmonata:
Lymnaeidae)]. Parazitologiya [Parasitology], 1999, Vol. 33, no. 2, pp. 125-128 (In Russian).
8. Dromashko S.E., Shevcova S.N. Biotestirovanie – sostavnoj element ocenki sostoyaniya okruzhayushchej sredy: uchebno-metodicheskoe posobie [Biotesting is an integral element of assessing the state of the environment: teaching aid]. Minsk, IPNK, 2012, 82 p.
9. Chirkin A.A., Docenko E.A., Yupatov G.I. Lipidnyj obmen [Lipidnyi obmen]. Moskva, Medicinskaya literatura, 2003, 122 p.
10. Lowry O.N. Protein measurement with Folin phenol reagent. Journal Biological Chemistry, 1951, Vol. 193, no. 1, pp. 265-275.
11. Blober G. Distribution of radioactivity between the acid-soluble pool and pools of RNA in the nuclear, non sedimentable and ribosome fractions of rat liver after a single injection of labeled orotic acid. Biochem. Biophys. Acta, 1968, Vol. 166, pp. 48-54.
12. Kuramshina, N.G., Kuramshin, E.M., Abroshchenko, V.V., Kirajd R.I. Biohimicheskie osobennosti plazmy gastropod (Mollusca: Gastropoda) v usloviyah razlichnyh urovnej tekhnogennoj nagruzki na territorii Bashkortostana [Biochemical features of gastropod plasma (Mollusca: Gastropoda) under different
levels of anthropogenic stress in the territory of Bashkortostan]. Materialy ІІІ Mezhdunarodnoj nauchnoj konferencii «Bioraznoobrazie i rol' zoocenozov v
prirodnyh i antropogennyh ehkosistemah» [Materials of the 3rd International Scientific Conference "Biodiversity and the role of zoocenoses in natural and anthropogenic ecosystems"]. Dnipropetrovsk, 2005, pp. 46-47 (In Russian).
