Анализ изменчивости количественных признаков у регенерантов Cupressus macrocarpa in vitro

Authors

  • Т.П. Кунаховец (Кunakhovec Т.P.)
  • В.В. Самойлович (Samoilovich V.V.)
  • О.А. Кудряшова (Kudryashova O.A.)
  • А.А. Волотович (Volotovich A.A.)
  • Л.А. Жизневская (Zhiznevskaya L.А.)
  • О.Ю. Артемчук (Artemchuk О.U.)

Abstract

В настоящей статье приведены результаты анализа изменчивости четырех количественных признаков у регенерантов кипариса крупноплодного Сupressus macrocarpa Hartw. & Gordon in vitro на питательных, агаризованных средах с органическими соединениями, на макро-, микросолевой основе Андерсона, различающихся по составу ауксинов (ИУК и ИМК) и цитокинина (зеатин). Достоверные (в большинстве случаев при Р<0,01) отличия по сравнению с контролем наблюдались только в вариантах опыта с 0,1 мг/л зеатинa. При этом в зависимости от варианта опыта, количество побегов увеличивалось достоверно в 1,3–2,0 раза; сырой вес регенерантов увеличивался в 1,6–2,2 раза. Высота регенерантов достоверно (при Р<0,05) уменьшалась в 1,2 раза в присутствии 0,1 мг/л зеатина и ИМК в концентрациях 0,1–0,5 мг/л. Установлены тенденции изменчивости анализируемых признаков с ростом концентраций исследуемых фитогормонов. Одно-факторный дисперсионный анализ установил достоверное (при P<0,01) влияние фактора 'варианты опыта' на изменчивость количества побегов и сырого веса регенерантов с долями влияния фактора 92% и 73%, соответственно. = In the present article are given the results of the analysis of variability of four quantitative traits at regenerants of Monterey cypress Cupressus macrocarpa Hartw. & Gordon in vitro on nutrient, agarized Anderson's mediums with organic compounds, on macro- and microsalt basis differing on structure of auxins (IAA and IBA), and cytokinin zeatin. Reliable differences in most cases at P<0.01 were observed only in experience variants with zeatin of 0.1 mg per liter in comparison with control. Thus, depending on experience variant the number of shoots increased authentically by 1.3–2.0 times; the crude weight of regenerants increased by 1.6–2.2 times. Height of regenerants authentically (at P<0.05) decreased by 1.2 times in the presence of 0.1 mg/l of zeatin and IBA in concentration of 0.1–0.5 mg/l. Tendencies of varia-bility of analyzed traits with growth of concentration of studied phytohormones are established. The onefactorial dispersive analysis established reliable (at P<0.01) 'experience variants' factor influence on variability of number of shoots and crude weight of regenerants with shares of influence of factor of 92% and 73%, respectively.

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Issue

Section

Biological sciences