The Influence of Nickel Sulfate on Biomass Yield and Protein Content in Chlorella Vulgaris in the Dynamics of Culture Growth
Keywords:
chlorella, culture, nickel sulfate, biomass, cellular protein, proteolytic activityAbstract
The addition of NiSO4 to the Chlorella vulgaris IBCE C-19 nutrient medium sat a concentration of 10–4…10–1 M caused inhibition of algal growth after a day, and at a concentration of ≥ 10–2 М – the death of culture after 13 days. However, the death of the chlorella culture was not observed at this effector concentration more than 5 times higher than that indicated in the publications (10 mg/l). At nickel sulfate concentration of 10–3 M after 5 and 9 days, the biomass level exceeded the values of the control variant by 16% and 19%, respectively. Even after 19 days, the biomass yield of this experimental case was deeply different from control by no more than 10%. Intracellular protein concentration shifts were of complex nature. However, changes in its level were not fatal and were not directly caused by shifts (depression ≤ 18%) in the activity of neutral intracellular caseinolytic and gelatinolytic proteinases.
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