Effect of inorganic orthophosphate on the physiological and biochemical state of cells of microalgae Chlorella Vulgaris strain C 111 IBCE C-19 at a low manganese (II) chloride conctntration in the nutrient medium
Keywords:
chlorella culture, inorganic orthophosphate, manganese (II) chloride, biomass, intracellular protein, chlorophylls, carotenoidsAbstract
The nature of changes in the level of biomass, concentration of intracellular protein, chlorophylls a and b, carotenoids was studied during cultivation of chlorella for 21 days on a nutrient medium containing KH2PO4 at a concentration of 1.784–2.230 g / L against a background of MnCl2 at a concentration of 0.025 or 0.050 mg / L. It was found that during growth on all experimental variants of the nutrient medium, the chlorella culture was in a physiologically active state. With the growth of algae on the variant “MnCl2 0.050 mg / L + KH2PO4 1.784 g / L”, the biomass yield was, in principle, comparable to the basic Tamiya medium, and even surpassed it in the accumulation of intracellular protein.
References
Ильючик, И. А. Рост культуры хлореллы (Chlorella vulgaris) и накопление белка при добавлении MnCl2 в питательную среду / И. А. Ильючик, В. Н. Никандров // Весн. Палес. дярж. ун-та. Сер. прыродазн. навук. – 2018. – № 1. – С. 53–64.
Ильючик, И. А. Физиолого-биохимическое состояние клеток культуры Chlorella vulgaris штамма IBCE C-19 при росте на питательной среде с карбонатом аммония / И. А. Ильючик, Е. М. Кандыба, В. Н. Никандров // Весн. Палес. дярж. ун-та. Сер. прыродазн. навук. – 2019. – № 2. – С. 40–50.
Загоровская, В Новый белок. Готов ли российский рынок к альтернативным кормовым белкам? / В. Загоровская // Агротехника и технологии. – 2020. – № 1. – С. 50–54.
Ковалев, М. М. Прогнозирование развития белорусского агропромышленного комплекса до 2030 г. На фоне глобальных агротрендов / М. М. Ковалев, Е. А. Червякова // Журнал Белорус. гос. ун-та. Экономика. – 2017. – № 2. – С. 120–139.
Suman, G. Single cell protein production: A review / G. Suman [et al.] // Int. J. Curr. Microbiol. App. Sci. – 2015. – Vol. 4, № 9. – P. 251–262.
Ursu, A.-V. Extraction, fractionation and functional properties of proteins from the microalgae Chlorella vulgaris / A.-V. Ursu [et al.] // Bioresour Technology. – 2014. – Vol. 157. – P. 134–139.
Melis, A. Solar energy conversion efficiencies in photosynthesis: minimizing the chlorophyll antennae to maximize efficiency / A. Melis // Plant Sci. – 2009. – Vol. 177, iss. 4. – P. 272–280
Panahi, Y. Impact of cultivation condition and media content on Chlorella vulgaris composition / Y. Panahi [et al.] // Adv. Pharm. Bull. – 2019. – Vol. 9, iss. 2. – P. 182–194.
Guerra-Renteria, A. S. Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. vulgaris and P. aeruginosa / A. S. Guerra-Renteria [et al.] // Int. J. of Molecular Sci. – 2019. – Vol. 20, iss. 8. – P. 1–9.
Лукьянов, В. А. Прикладные аспекты применения микроводорослей в агроценозе / В. А. Лукьянов, А. И. Стифеев. – Курск : Изд-во Кур. гос. с.-х. акад., 2014. – 181 с.
Упитис, В. В Макро- и микроэлементы в оптимизации минерального питания микроводорослей / В.В. Упитис – М.: Рига, Зинатне, 1983. – 240 с.
Ильючик, И. А. Динамика фотосинтетических пигментов в культуре водоросли Chlorella vulgaris штамма С 111 IBCE C-19 при росте на питательной среде с добавлением хлорида марганца / И.А. Ильючик, В.Н. Никандров // Весцi Нац. акад. навук Беларусі. Сер. біял. навук. – 2020. – Т. 65, № 3. – С. 299–309.
Ильючик, И. А. О протеолитической активности субклеточных фракций клеток Chlorella vulgaris штамма С 111 IBCE C-19 / И.А. Ильючик, В.Н. Никандров // Доклады Нац. акад. наук Беларуси. – 2020. – Т. 64, № 6. – С. 694–701.
Pax C. Blamey, F. Manganese distribution and speciation help to explain the effects of silicate and phosphate on manganese toxicity in four crop species / F. Pax C. Blamey [et al.] //New Phytologist. – 2018. – Vol. 217. – Р. 1146–1160.
Каталог генетического фонда хозяйственно полезных видов водорослей: научное издание / Национальная академия наук Беларуси, Институт биофизики и клеточной инженерии; сост. С.С. Мельников [и др.]. – Минск: Беларуская навука. – 2011. – 101 с
Гавриленко, В. Ф. Большой практикум по фотосинтезу / В. Ф. Гавриленко, Т. В. Жигалова ; под ред. И. П. Ермакова. – М. : Изд. центр «Академия», 2003. – 256 с.
Елизарова, В. А. Содержание фотосинтетических пигментов в фитопланктоне водоемов разного типа : автореф. дис. канд. биол. наук : 03.00.18 / В. А. Елизарова ; Ин-т биологии внутренних вод АН СССР. – М., 1975. – 24 с.
Ильючик, И. А. Влияние неорганического ортофосфата на динамику накопления биомассы и внутриклеточного белка в культуре хлореллы (Chlorella vulgaris) / И. А. Ильючик, А. И. Лакишик, В. Н. Никандров // Биотехнология: достижения и перспективы развития: сб. матер. IV Междунар. научно-практ. конф., Пинск, 20–22 ноября 2019 г. – Пинск : ПолесГУ, 2019. – С. 52–57.
References
Il'iuchik I.A., Nikandrov V.N. Rost kulturyi сhlorellyi (Chlorella vulgaris) i nakoplenie belka pri dobavlenii MnCl2 v pitatelnuyu sredu [Chlorella vulgaris culture growth and protein accumulation at MnCl2 addition in nutrient medium]. Vesnik Paleskaga dzyarzhaunaga universiteta. Seryya pryrodaznauchykh navuk [Bulletin of Polessky State University. Series in Natural Sciences], 2018, no. 1, pp. 53–64. (In Russian)
Il'iuchik I.A., Kandyba E.M., Nikandrov V.N. Fiziologo-biohimicheskoe sostoyanie kletok kul'tury Chlorella vulgaris shtamma IBCE C-19 pri roste na pitatel'noj srede s karbonatom ammoniya [Physiology and biochemical state of Chlorella vulgaris IBCE C-19 cell during the culture growth on medium with ammonium carbonate]. Vesnik Paleskaga dzyarzhaunaga universiteta. Seryya pryrodaznauchykh navuk [Bulletin of Polessky State University. Series in Natural Sciences], 2019, no. 2, pp. 40–50. (In Russian)
Zagorovskaya V. Novyj belok. Gotov li rossiyskiy rynok k al'ternativnym kormovym belkam [New protein. Is the Russian market ready for alternative feed proteins?]. Agrotekhnika i tekhnologii [Agrotechnics and Technology], 2020, no. 1, pp. 50–54. (In Russian)
Kovalev M.M., Chervyakova E.A. Prognozirovanie razvitiya belorusskogo agropromyshlennogo kompleksa do 2030 g. Na fone global'nyh agrotrendov [Forecasting the development of the Belarusian agro-industrial complex until 2030 Against the background of global agricultural trends]. Zhurnal Belorusskogo. gosudarstvennogo universiteta. Ekonomika [Journal of the Belarusian State University. Economy], 2017, no. 2, pp. 120–139. (In Russian)
Suman G., Nupur M., Anuradha S., Pradeep B. Single cell protein production: A review. International Journal of Current Microbiology and Applied Sciences, 2015, vol. 4, no. 9, pp. 251–262.
Ursu A.-V. et al. Extraction, fractionation and functional properties of proteins from the microalgae Chlorella vulgaris. Bioresour Technology, 2014, vol. 157, pp. 134–139.
Melis, A. et al. Solar energy conversion efficiencies in photosynthesis: minimizing the chlorophyll antennae to maximize efficiency. Plant Science, 2009, vol. 177, iss. 4, pp. 272–280.
Panahi Y., Khosroushahi A.Y, Sahebkar A., Heidari H.R. Impact of cultivation condition and media content on Chlorella vulgaris composition. Advanced Pharmaceutical Bulletin, 2019, vol. 9, iss. 2, pp. 182–194.
Guerra-Renteria A.S. et al. Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. vulgaris and P. aeruginosa. International Journal of Molecular Sciences, 2019, Vol. 20, iss. 8, pp. 1–9.
Luk'yanov V.A., Stifeev A.I. Prikladnye aspekty primeneniya mikrovodoroslej v agrocenoze [Applied aspects of the use of microalgae in agrocenosis]. Kursk: Izdatel'stvo Kurskoj gosudarstvennoj sel'skohozyajstvennoj akademii, 2014. 181 p. (In Russian)
Upitis V.V. Makro- i mikroelementy v optimizatsii mineral'nogo pitaniia mikrovodoroslei [Macro and microelements in the optimization of mineral nutrition of microalgae]. Riga, Zinatne Publ., 1983. 240 p. (In Russian)
Il'iuchik I.A., Nikandrov V.N. Dinamika fotosinteticheskih pigmentov v kul'ture vodorosli Chlorella vulgaris shtamma C 111 IBCE C-19 pri roste na pitatel'noj srede s dobavleniem hlorida marganca [Dynamics of a photosynthetic pigments level of the Chlorella vulgaris strain С 111 IBCE C-19 during the growth at the nutrien medium with manganese chloride addition]. Vesci Nacional'noу akademii nauk Belarusі. Seriya bіyalagichnyh nauk [Proceedings of the National Academy of Sciences of Belarus. Biological Series]. – 2020. – T. 65, № 3. – S. 299–309. (In Russian).
Il'iuchik I.A., Nikandrov V.N O proteoliticheskoj aktivnosti subkletochnyh frakcij kletok Chlorella vulgaris shtamma S 111 IBCE C-19 [On proteolytic activity of subcellular fractions of Chlorella vulgaris strain C 111 IBCE C-19 cell]. Doklady Nacional'noу akademii nauk Belarusі [Reports of the National Academy of Sciences of Belarus] – 2020. – T. 64, № 6. – S. 694–701. (In Russian).
Pax C. Blamey F. [et al.] Manganese distribution and speciation help to explain the effects of silicate and phosphate on manganese toxicity in four crop species. New Phytologist, 2018, vol. 217, pp. 1146–1160.
Mel'nikov S.S. et al. Katalog geneticheskogo fonda khoziaistvenno poleznykh vidov vodoroslei [Catalog of the genetic fund of economically useful species of algae]. Minsk, Belarusskaia navuka Publ, 2011. 101 р. (In Russian).
Gavrilenko V.F. Bol'shoj praktikum po fotosintezu [Large workshop on photosynthesis]. Ed. Ermakova I.P. Moscow. Izdatel'skij centr «Akademiya», 2003, 256 р. (in Russian).
Elizarova V.A. Soderzhanie fotosinteticheskih pigmentov v fitoplanktone vodoemov raznogo tipa [The content of photosynthetic pigments in phytoplankton of reservoirs of various types]. Abstract of Ph. D. thesis. Moscow, 1975, 24 p. (In Russian).
Il'iuchik I.A., Lakishik A.I., Nikandrov V.N. Vliyanie neorganicheskogo ortofosfata na dinamiku nakopleniya biomassy i vnutrikletochnogo belka v kul'ture сhlorelly (Chlorella vulgaris) [Influence of inorganic orthophosphate on the dynamics of biomass and intracellular protein accumulation in the culture of chlorella (Chlorella vulgaris)] Biotekhnologiya: dostizheniya i perspektivy razvitiya: sbornik materialov IV mezhdunarodnoj nauchno-prakticheskoj konferencii, Pinsk, Respublika Belarus', 20–22 noyabrya 2019 g., Polesskij gosudarstvennyj universitet [Biotechnology: Achievements and Development Prospects: Collection of Materials of the IV International Scientific and Practical Conference, Pinsk, Republic of Belarus, November 20-22, 2019, Polessky State University.] 2019, pp. 52–57. (In Russian).
