Влияние бетулина на активность карнитин-пальмитоилтрансферазы1 типа в митохондриях печени крыс

A.H. Shlyahtun; Yu.Z. Maksimchyk; E.F. Raduta; I.P. Sutsko

Authors

A.H. Shlyahtun Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus
Yu.Z. Maksimchyk Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus
E.F. Raduta Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus
I.P. Sutsko Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus

Keywords:

betulin, carnitine palmitoyltransferase, liver, mitochondria, lipid metabolism

Abstract

The study on the effects of betulin on carnitine palmitoyltransferase 1 in rat liver mitochondria was performed. It was shown that betulin elevates the activity of enzyme in vivo. Strong negative correlation was found between carnitine palmitoyltransferase 1 activity in rat liver mitochondria and free fatty acids content in blood serum. Betulin had no direct effect on enzyme activity as it was demonstrated based on in vitro studies. It was concluded that upregulation of protein content or changes on the level of posttranslational modification may be related to the increased activity enzyme in rat liver mitochondria.

Author Biographies

A.H. Shlyahtun, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus

Head of the laboratory

Yu.Z. Maksimchyk, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus

Senior Researcher

E.F. Raduta, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus

Senior Researcher

I.P. Sutsko, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus

PhD in Biol. Sc., Senior Researcher

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Buko V. et al. Betulin attenuated liver damage by prevention of hepatic mitochondrial dysfunction in rats with alcoholic steatohepatitis. Physiology International, 2019, vol. 106, iss. 4, pp. 323–334. doi: 10.1556/2060.106.2019.26.

Bonnefont J. P. et al. Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspects. Mol. Aspects Med., 2004, vol. 25, iss. 5–6, pp. 495–520. doi: 10.1016/j.mam.2004.06.004.

Schlaepfer I. R., Joshi M. CPT1A-mediated fat oxidation, mechanisms, and therapeutic potential. Endocrinology, 2020, vol. 161, iss. 2, article ID. bqz046. doi: 10.1210/endocr/bqz046.

Buko V. et al. Betulin/2-hydroxypropyl-β-cyclodextrin inclusion complex: physicochemical characterization and hepatoprotective activity. J. Mol. Liq., 2020, vol. 309, article ID. 113118. doi: 10.1016/j.molliq.2020.113118.

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Johnson D., Lardy H. Isolation of liver or kidney mitochondria. Methods Enzymol., 1967, vol. 10, pp. 94–96. doi: 10.1016/0076-6879(67)10018-9.

Bieber L. L., Abraham T., Helmrath T. A rapid spectrophotometric assay for carnitine palmitoyltransferase. Anal. Biochem., 1972, vol. 50, iss. 2, pp. 509–518. doi: 10.1016/0003-2697(72)90061-9.

Peterson G. L. Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall. Anal. Biochem., 1979, vol. 100, iss. 2, pp. 201–220.

Jäger S., Laszczyk M. N., Scheffler A. A preliminary pharmacokinetic study of betulin, the main pentacyclic triterpene from extract of outer bark of birch (Betulae alba cortex). Molecules, 2008, vol. 13, iss. 12, pp. 3224–3235. doi: 10.3390/molecules13123224.

Diniz M. F. et al. Haematological and biochemical parameter standardization of Swiss mice and Wistar rats. Rev. Bras. Cienc. Saude., 2006, vol. 10, iss. 2, pp. 171–176.