Engraftment and rejection of skin-cartilaginous xenografts transplanted to rats from spiny mouse
Keywords:
rats, spiny mouse (acomys), auricle, skin-cartilaginous xenografts, chondrocytes, xenotransplantationAbstract
Purpose of the study. The aim of the study was to reveal by means of morphological methods the peculiarities of engraftment and rejection of skin-cartilaginous xenografts made from Acomys auricles (spiny mice) and transplanted to the surface of a full-layer skin defect in laboratory rats.
Materials and Methods. 30 female Wistar rats weighing 210-250 g were used as recipients, 9 adult Acomys were used as transplant donors. In the recipients the hair was removed in the interscapular region and the plastic security fixation was sutured. A full-thickness skin defect was created on the skin area limited by the camera. In Acomys, the donors of xenografts, the auricles were cut off, from the outer side of which the skin was removed, and from the skin of the inner surface, containing a layer of chondrocytes, an area of 1 cm2 was cut out from which smaller grafts were made. Each rat had 1-2 xenografts placed on the receptive bed. Recipient rats were withdrawn from the experiment after transplantation after 3 days-30 days. A rectangular strip of tissue taken from the receptive bed was used for histologic studies.
Results. As a result of the conducted research it was established that skin-cartilaginous xenografts undergo two stages in their development – engraftment (1-5 days) and rejection (after 5 days). In the course of rejection, the epidermal-dermal component of such a graft is destroyed first, and then – the layer of chondrocytes, which initially may be immersed in the granulation tissue of the receptive bed.
References
Cooper, D. K. C. Xenotransplantation—the current status and prospects / D. K. C. Cooper [et al.] // British Medical Bulletin. – 2017. – Vol. 125(1). – P. 5–14. DOI:10.1093/bmb/ldx043.
Lu, T. Xenotransplantation: Current Status in Preclinical Research / T. Lu [et al.] // Frontiers in Immunology. – 2020. – Vol. 10. doi:10.3389/fimmu.2019.03060.
Zhang, Z. Animal models in xenotransplantation. Expert Opinion on Investigational / Z. Zhang [et al.] // Drugs. – 2020. – Vol. 9(9). – P. 2051–2068. DOI:10.1517/13543784.9.9.2051
Burdick, J. Variations in the responses of mouse strains to rat xenografts / J. Burdick, S. Jooste, Н. Winn // J Immunol. – 1979. – Vol. 123 (2). – P. 954–955.
Радута, А. Ф. Трансплантацыйны патэнцыял ліпахандрацытаў вушной ракавіны лабараторных пацукоў / А. Ф. Радута [і інш.] // Биохимия и молекулярная биология. Выпуск 4 : сб. статей, посвященный 95-летию основателя Института биохимии биологически активных соединений Национальной академии наук Беларуси академика Ю. М. Островского / Минск, ИВЦ «Минфина» ; гл. ред. Семененя И.Н. д.м.н., проф. – Гродно, 2020. – С. 173–177.
Астроўскі, А. А. Асаблівасці прыжыўлення і адрынання вушных алатрансплантатаў у лабараторных пацукоў / А. А. Астроўскі [і інш.] // Известия Национальной академии наук Беларуси. Серия медицинских наук. – 2021. – Т. 18, № 4. – С. 422–432. DOI.org/10.29235/1814-6023-2021-18-4-422-432.
Maden, M. Model systems for regeneration: the spiny mouse, Acomys cahirinus / M. Maden, J. A. Varholick // Development – 2020. – Т. 147, № 4. dev167718. DOI:10.1242/dev.167718.
Gaire, J. Spiny mouse (Acomys): an emerging research organism for regenerative medicine with applications beyond the skin / J. Gaire [et al.] // NPJ Regenerative Medicine. – 2021. – 4;6(1):1. DOI: 10.1038/s41536-020-00111-1.
Allen, R. S. Neural crest cells give rise to non-myogenic mesenchymal tissue in the adult murid ear pinna / R. S. Allen, Sh. K. Biswas, A. W. Seifert // bioRxiv. – 2023. DOI: 10.1101/2023.08.06.552195.
Руководство по содержанию и уходу за лабораторными животными. Правила оборудования помещений организации процедур : ГОСТ 33215-2014. – М. : Стандартинформ, 2019. – 12 с.
Руководство по содержанию и уходу за лабораторными животными. Правила содержания и ухода залабораторными грызунами и кроликами : ГОСТ 33216-2014. – М. : Стандартинформ, 2019. – 9 с.
Надлежащая лабораторная практика : ТКП 125-2008 (02040). – Минск : М-во здравоохранения Респ. Беларусь, 2008. – 35 с.
Бакуновіч, А. А. Эксперыментальная мадэль для ацэнкі гатоўнасці ранавай паверхні да прыняцця скурных трансплантатаў / А. А. Бакуновіч [і інш.] // Известия Национальной академии наук Беларуси. Серия медицинских наук /– 2021. – Т. 18, № 3. – С. 340–350. https://doi.org/10.29235/1814-6023-2021-18-3-340-350.
Астроўскі, А. А. Комплекснае макраскапічнае, гісталагічнае і электронна-мікраскапічнае даследаванне загойвання паўнаслойнай скурнай раны ў лабараторных пацукоў / А. А. Астроўскі, А. А. Бакуновіч, А. Б. Астроўская // Известия Национальной академии наук Беларуси. Серия медицинских наук. – 2022. – Т. 19, № 3. – С. 278–289. https://doi.org/10.29235/1814-6023-2022-19-3-278-289.
References
Cooper D. K. C. et al. Xenotransplantation—the current status and prospects. British Medical Bulletin; 2017; 125(1):5–14. DOI:10.1093/bmb/ldx043.
Lu T. et al. Xenotransplantation: Current Status in Preclinical Research. Frontiers in Immunology; 2020:10. DOI:10.3389/fimmu.2019.03060.
Zhang Z. [et al.] Animal models in xenotransplantation. Expert Opinion on Investigational. Drugs, 2020; 9(9): 2051–2068. doi:10.1517/13543784.9.9.2051.
Burdick J., Jooste S., Winn Н. Variations in the responses of mouse strains to rat xenografts. J Immunol, 1979; 123 (2): 954–955.
Raduta A.F. Transplantacyjny patencyyal lіpahandracytaў vushnoj rakavіny labaratornyh pacukoў [Transplantation potential of auricle lipochondrocytes of laboratory rats]. Biochemistry and molecular biology. Issue 4, a collection of articles devoted to the 95th anniversary of the founder of the Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Academician Yu. M. Ostrovsky, see Ed. Semenenia I. N. Doctor of Medicine, Prof. Minsk, ITC "Ministry of Finance", 2020:173–177.
Astroўskі A.A. Asablіvascі pryzhyўlennya і adrynannya vushnyh alatransplantataў u labaratornyh pacukoў [Features of engraftment and rejection of ear alatransplants in laboratory rats] // Proceedings of the National Academy of Sciences of Belarus, Medical series, 2021;18(4):422-432. (In Bel.) DOI.org/10.29235/1814-6023-2021-18-4-422-432
Maden M., Varholick J.A. Model systems for regeneration: the spiny mouse, Acomys cahirinus. Development, 2020;147(4), dev167718. DOI:10.1242/dev.167718
Gaire J. Spiny mouse (Acomys): an emerging research organism for regenerative medicine with applications beyond the skin. npj Regenerative Medicine, 2021; 4;6(1):1.
Allen R.S., Biswas Sh.K., Seifert A.W. Neural crest cells give rise to non-myogenic mesenchymal tissue in the adult murid ear pinna. bioRxiv. 2023 DOI: 10.1101/2023.08.06.552195.
Rukovodstvo po soderzhaniyu i uhodu za laboratornymi zhivotnymi. Pravila oborudovaniya pomeshchenij organizacii procedur [State Standart 33215-2014. Guidelines for the maintenance and care of laboratory animals. Rules for equipping premises and organizing procedures], Moscow, Standartinform Publ., 2019. 12 p. (in Russian).
Rukovodstvo po soderzhaniyu i uhodu za laboratornymi zhivotnymi. Pravila oborudovaniya pomeshchenij organizacii procedur : GOST 33215-2014. [State Standart 33216-2014. Guidelines for the maintenance and care of laboratory animals. Rules for the maintenanceand care of laboratory rodents and rabbits.]. Moscow, Standartinform Publ., 2019. 9 p. (in Russian)
Nadlezhashchaya laboratornaya praktika : TKP 125-2008 (02040) [TKP 125-2008 (02040). Good Laboratory Practice. Minsk. Ministry of Health of the Republic of Belarus], Moscow, Standartinform, 2008. 35 p. (in Russian).
Bakunovіch A.A. Eksperymental'naya madel' dlya acenkі gatoўnascі ranavaj paverhnі da prynyaccya skurnyh transplantataў Izvestiya Nacional'noj akademii nauk Belarusi. Seriya medicinskih nauk [Experimental model for assessing the readiness of the wound surface to accept skin grafts]. Proceedings of the National Academy of Sciences of Belarus, Medical series, 2021;18(3):340-350. (In Bel.) https://doi.org/10.29235/1814-6023-2021-18-3-340-350.
strowski A.A. Kompleksnae makraskapіchnae, gіstalagіchnae і elektronna-mіkraskapіchnae dasledavanne zagojvannya paўnaslojnaj skurnaj rany ў labaratornyh pacukoў [Complex macroscopic, histological, and electron microscopic examination of the healing of a full-thickness skin wound in laboratory rats]. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2022;19(3):278-289. (In Bel.) https://doi.org/10.29235/1814-6023-2022-19-3-278-289
