Effect of copper sulphate on the physiological and biochemical state of cells of Chlorella Vulgaris
Keywords:
chlorella culture, copper sulfate, biomass level, intracellular protein, chlorophyllsAbstract
When adding CuSO4 to the Tamiya medium in the concentration range of 10–8–10–4 M, the Chlorella vulgaris culture retained its viability for 21 days. However, at all concentrations of copper sulfate, in comparison with the control, inhibition of biomass growth was noted, especially after the 9th day. The maximum effect - a decrease of 34–53% - was revealed at a Cu2+ concentration of 10–4 M. Changes in the concentration of intracellular protein in all variants of the nutrient medium were close to the biomass dynamics. At the same time, adding CuSO4 to the nutrient medium in concentrations of 10–8 and 10–7 M stimulated the accumulation of intracellular protein in relation to the control up to the 11th day, and on the 7th day these variants of the nutrient medium exceeded the control by 20%. The introduction of CuSO4 into the nutrient medium was accompanied by a decrease in the level of chlorophyll a accumulation by chlorella cells, and the higher the concentration of the effector in the nutrient medium, the more pronounced it was. Changes in the level of chlorophyll b in cells throughout the observation period were less pronounced compared to those of chlorophyll a. In the dynamics of chlorella culture growth, the level of carotenoids in its cells increased, especially pronounced at a CuSO4 concentration of 10−4 and 10−5 M: the level of pigments on the 15th–17th day increased by 2.3–2.7 times compared to the beginning of cultivation.
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