Выявление генетического родства представителей рода Turbinicarpus Buxb. & Backeb. (Cactaceae) с использованием молекулярных IPBS-маркеров

T.G. Kulahina

Authors

T.G. Kulahina Central Botanical Garden National Academy of Sciences of Belarus, Minsk, Republic of Belarus

Keywords:

cluster analysis, species relationships, dendrogram, cacti

Abstract

Objective. To determine the genetic relationships among representatives of the genus Turbinicarpus Buxb. Backeb. based on cluster analysis.

Materials and Methods. Molecular-genetic analysis using iPBS markers, cluster analysis of genetic dendrograms, and comparative analysis of genetic and morphological dendrograms.

Results. The cluster analysis of Turbinicarpus species revealed complex interrelationships among them and identified three main clusters, consisting of 2, 6, and 33 species, respectively. Special attention should be given to the third cluster, which contains the largest number of species, including a previously unidentified taxon that is genetically close to T. mandragora. The comparison of the obtained genetic and morphological dendrograms shows both similarities and differences with data from other researchers, highlighting the complexity of the systematics of this genus and the need for further studies.

Conclusion. The conducted research complements previously known information about the genetic relationships among Turbinicarpus species and emphasizes the importance of a comprehensive approach to analyzing the systematics and evolution of species, as well as the necessity of considering various factors.

Author Biography

T.G. Kulahina, Central Botanical Garden National Academy of Sciences of Belarus, Minsk, Republic of Belarus

Scientific Employee of the Sector of the Conservation and Restoration of Plant Resources

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References

Hunt D.R., Taylor N.P, Charles G. The New Cactus Lexicon. International Cactaceae Systematics Group. Milborne Port, Dh Books, 2013, 527 p.

Vázquez-Sánchez M., Terrazas T., Arias S. H. Ochoterena Molecular phylogeny, origin and taxonomic implications of the tribe Cacteae (Cactaceae). Systematics and Biodiversity. 2013. Vol. 11, no.1, pp. 103–116.

Vázquez-Sánchez M., Terrazas T., Arias S. El hábito y la forma de crecimiento en la tribu Cacteae (Cactaceae, Cactoideae). Botanical Sciences. 2012. Vol. 90, pp. 97–108.

Hernández-Hernández T., Brown J.W., Schlumpberger B.O., Eguiarte L.E., Magallón S. Beyond aridification: multiple explanations for the elevated diversification of cacti in the New World succulent biome. The New Phytologist. 2014. Vol. 202, pp. 1382–1397.

Glass C., Foster R. A revision of the genus Turbinicarpus (Backbg.) Buxb. & Backbg. Cactus and Succulent Journal. 1977.Vol. 49, pp. 161–176.

Buxbaum F., Backeberg C. Cactaceae. Jahrbücher der Deutschen Kakteen-Gesellschaft. Cactaceae Jahrbücher der Deutschen Kakteengesellschaft E. V. Glückstadt, 1937, 27 p.

Bravo-Hollis H., Sánchez-Mejorada H. Las cactáceas de México. Mexico City, Universidad Nacional Autónoma de México. 1991. Vol. 2, 404 p.

Doweld A.B. 1998. An outline of the phylogenetic system of classification of the tribe Cacteae (Cactoideae – Cactaceae). I. System of the subtribes Sclerocactinae – Bravocactinae - Turbinicarpinae. Succulents. Moscow 1998. Vol.1, pp. 15–30.

Anderson E.F. The Cactus Family. First Edition. Portland, Or, Timber Press, Incorporated, 2001, 776 p.

Guzmán U., Arias S., Dávila P. Catálogo de cactáceas mexicanas. Mexico City: Universidad Nacional Autónoma de México y Comisión Nacional para el Conocimiento y Uso de la Biodiversidad, 2003, 315 p.

Hunt D.R. 2016. CITES Cactaceae checklist, 3rd edition. Kew, Royal Botanic Gardens, 2016, 315 p.

Backeberg C. Cactaceae Lindle. Systematiс synopsis. Blätter fur Kakteenforfchung. 1938. Jahgr.5, Pt. (unpaged).

Backeberg C., Haage W., Glass L.. Cactus lexicon. Enumeratio diagnostica cactacearum London, Blandford Press, 1977, 828 p.

Buxbaum F., Oehme H. Cactaceae. Jahrbücher der Deutschen Kakteen-Gesellschaft. Neudamm, Deutschen Kakteengesellschaft E. V., 1942, pp.18–24.

Lüthy J.M. The Turbinicarpus mandragora complex. Bradleya. 2001. Vol. 19, pp. 19–54.

Lüthy J. M. Further comments on Turbinicarpus and a key to species. Cactaceae Systematics Initiatives. 2002. Vol. 14, pp. 21–25.

Lüthy J.M. Rapicactus Buxbaum & Oehme: revision of the genus. Cactus & Co. 2003. Vol. 7, pp. 4–43.

Anderson E.F. A revision of the genus Neolloydia B.&R. (Cactaceae). Bradleya. 1986. Vol. 4, pp. 1–28.

Bravo-Hollis H., Marshall W.T. A revision of the genus Toumeya. Saguaroland Bulletin. 1956. Vol. 10, pp. 112–119.

Halda J. J. A new system of the genus Pediocactus Britton & Rose. Acta Musei Richnoviensis Section Nature. 1998. Vol. 5, pp. 1–48.

Kalendar R. et al. iPBS: a universal method for DNA fingerprinting and retrotransposon isolation. TAG Theor. Appl. Genet. Theor. Angew. Genet. 2010. Vol. 121, no.8, pp. 1419–1430.

Vázquez-Sánchez M. et al. Polyphyly of the iconic cactus genus Turbinicarpus (Cactaceae) and its generic circumscription. Botanical Journal of the Linnean Society. 2019. Vol. 190, pp. 405–420.

Shlapakova T.G., Titok V.V. Morfologicheskie osobennosti semyan roda Turbinicarpus [Morphological features of seeds of the genus Turbinicarpus]. Vesci Nacyyanal'naj akademii navuk Belarusі. Seryya bіyalagichnyh navuk [News of the National Academy of Sciences of Belarus. Series of biological sciences.], 2021. Vol. 66, no 4, pp. 482–490. (In Russian)