Study of polymorphism pattern of the pronotum of imago Leptinotarsa decemlineata Say, 1824 and variability of CYP450 gene families as mechanisms of resistance to insecticides
Keywords:
resistance, insecticides, preparation «Bitoxibacillin», phenoforms, CYP450 system, variabilityAbstract
In L. decemlineata populations collected in the Brest region, all the pronotum pattern phenoforms described by Fasulati were observed in adults with varying frequencies. A comparative analysis of the phenotypic structure of the studied L. decemlineata populations revealed that areal separated populations are characterized by similarities in the central pronotal pattern. The frequency of occurrence varies between years, possibly due to the use of different groups of insecticides.
Different phenoforms exhibit varying levels of resistance to «Bitoxibacillin», indicating the need for ongoing monitoring and selection of effective insecticides for agricultural use, taking into account evolutionary changes in L. decemlineata populations. In L. decemlineata adults, the following genes are expressed in high copy numbers and exhibit high variability: CYP4BN13, CYP4C1, CYP4C3, CYP6A23, and CYP6K1LIKE. Since exposure to insecticides influences copy number variation increases, it can be assumed that the analyzed L. decemlineata adults were exposed to insecticides, what triggered their gene expression.
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