Metabolic precursors of NAD biosynthesis in the prevention of alcohol-induced liver steatosis in rats
Keywords:
alcoholic liver disease, steatosis, hepatoprotectant, nicotinamide adenine dinucleotide, nicotinamide, nicotinamide riboside, nicotinamide mononucleotide, lipid metabolismAbstract
It has been shown that precursors of NAD biosynthesis restore key parameters of lipid metabolism in chronic alcohol intoxication and inhibit the progression of alcohol-induced hepatic steatosis in rats.
References
Alcohol-associated liver disease - Global epidemiology / F. Åberg, Z. G. Jiang, H. Cortez-Pinto, V. Männistö // Hepatology. – 2024. – Vol. 80, № 6. – P. 1307–1322. DOI: 10.1097/HEP.0000000000000899.
French, S. W. Chronic alcohol binging injures the liver and other organs by reducing NAD⁺ levels required for sirtuin's deacetylase activity / S. W. French // Exp. Mol. Pathol. – 2016. – Vol. 100, № 2. – P. 303–306. DOI: 10.1016/j.yexmp.2016.02.004.
Chen, M. Alcohol and the mechanisms of liver disease / M. Chen, W. Zhong, W. Xu // J. Gastroenterol. Hepatol. – 2023. – Vol. 38. – P. 1233–1240. DOI: 10.1111/jgh.16282.
Pharmacological interventions for alcoholic liver disease (alcohol-related liver disease): an attempted network meta-analysis / E. Buzzetti, M. Kalafateli, D. Thorburn [et al.] // Cochrane Database Syst Rev. – 2017. – Vol. 3, № 3. – Art. CD011646. doi: 10.1002/14651858.CD011646.pub2.
ACG Clinical Guideline: alcohol-associated liver disease / L. L. Jophlin, A. K. Singal, R. Bataller [et al.] // Am. J. Gastroenterol. – 2024. – Vol. 119, № 1. – P. 30–54. DOI: 10.14309/ajg.0000000000002572.
Шляхтун, А.Г. Влияние профилактического введения предшественников биосинтеза никотинамидадениндинуклеотида на активность систем метаболизма спиртов и альдегидов в печени крыс при острой тяжелой алкогольной интоксикации / А.Г. Шляхтун, Е. В. Букша, Е. В. Богдевич [и др.] // Физ.-хим. биол. как основа совр. медицины : докл. междунар. науч. конф., посвящ. 75-летию со дня рождения проф. Е. В. Барковского, Минск, 28 октября 2022 г. / под ред. В. В. Хрусталёва, А. Д. Тагановича, Т. А. Хрусталёвой. – Минск, 2022. – С. 377–382.
Шляхтун, А. Г. Протекторное действие предшественников биосинтеза никотинамидадениндинуклеотида на функциональное состояние митохондрии печени при алкогольной интоксикации / А. Г. Шляхтун, И. П. Сутько, Е. В. Букша [и др.] // Биохимия и молекулярная биология. – 2023. – Т. 2, № 3. – С. 14–21.
Restoring energy metabolism by NAD+ supplement prevents alcohol-induced liver injury and boosts liver regeneration / Y. Liu, C. Cheng, H. Gao [et al.] // Food Sci. Nutr. – 2024. – Vol. 12, № 7. – P. 5100–5110. DOI: 10.1002/fsn3.4159.
β-Nicotinamide mononucleotide alleviates alcohol-induced liver injury in a mouse model through activation of NAD+/SIRT1 signaling pathways / X. Yang, E. Zheng, Y. Lin [et al.] // Hereditas. – 2025. – Vol. 162, № 1. – Art. 161. doi: 10.1186/s41065-025-00529-x.
Liu, R. A novel preparation of nicotinamide mononucleotide / R. Liu, J. Visscher // Nucleosides Nucleotides. – 1994. – Vol. 13, № 5. – P. 1215–1216. DOI: 10.1080/15257779408011891.
Recommendations for euthanasia of experimental animals: Part 1. DGXI of the European Commission / B. Close, K. Banister, V. Baumans [et al.] // Lab Anim. – 1996. – Vol. 30, № 4. – P. 293–316. DOI:10.1258/002367796780739871.
Duncombe, W. G. The colorimetric micro-determination of non-esterified fatty acids in plasma / W. G. Duncombe // Clinica Chim. Acta. – 1964. – Vol. 9. – P. 122–125. DOI: 10.1016/0009-8981(64)90004-x.
Folch, J. A simple method for the isolation and purification of total lipides from animal tissues / J. Folch, M. Lees, S. G. H. Sloane // J. Biol. Chem. – 1957. – Vol. 226, № 1. – P. 497–509.
Fatty acid composition of liver total lipids in alcoholic patients with and without liver damage / M. P. de la Maza, S. Hirsch, S. Nieto [et al.] // Alcohol Clin. Exp. Res. – 1996. – Vol. 20, № 8. – P. 1418–1422. DOI: 10.1111/j.1530-0277.1996.tb01143.x.
Buko, V. U. Role of microsomal ethanol-oxidizing system in regulation of linoleoyl-CoA desaturase activity after long-term ethanol administration / V. U. Buko, L. I. Sushko // Alcohol Alcohol. – 1988. – Vol. 23. – № 1. – P. 69–71.
References
Åberg F., Jiang Z.G., Cortez-Pinto H., Männistö V. Alcohol-associated liver disease-Global epidemiology. Hepatology, 2024, vol. 80, iss. 6, pp. 1307–1322. DOI: 10.1097/HEP.0000000000000899
French S.W. Chronic alcohol binging injures the liver and other organs by reducing NAD⁺ levels required for sirtuin's deacetylase activity. Exp. Mol. Pathol., 2016, vol. 100, iss. 2, pp. 303–306. DOI: 10.1016/j.yexmp.2016.02.004
Chen M., Zhong W., Xu W. Alcohol and the mechanisms of liver disease. J. Gastroenterol. Hepatol., 2023, vol. 38, pp. 1233–1240. DOI: 10.1111/jgh.16282
Buzzetti E., Kalafateli M., Thorburn D., et al. Pharmacological interventions for alcoholic liver disease (alcohol-related liver disease): an attempted network meta-analysis. Cochrane Database Syst Rev., 2017, vol. 3, no. 3, article CD011646. DOI: 10.1002/14651858.CD011646.pub2
Jophlin L.L., Singal A.K., Bataller R., et al. ACG Clinical Guideline: alcohol-associated liver disease. Am. J. Gastroenterol., 2024, vol. 119, iss. 1, pp. 30–54. DOI: 10.14309/ajg.0000000000002572
Shlyahtun A.H., Buksha E.V., Bogdevich E.V., et al. Vliyanie profilakticheskogo vvedeniya predshestvennikov biosinteza nikotinamidadenindinukleotida na aktivnost` sistem metabolizma spirtov i al`degidov v pecheni kry`s pri ostroj tyazheloj alkogol`noj intoksikaczii [The effect of prophylactic administration of nicotinamide adenine dinucleotide biosynthesis precursors on the activity of alcohol and aldehyde metabolism systems in rat liver during acute severe alcohol intoxication]. Fiz.-khim. biol. kak osnova sovr. medicziny` [Physico-chemical biology as the basis of modern medicine: abstracts of reports of participants of the republican conference with international participation]. Eds. Khrustalev V.V., Taganovich A.D., Khrustaleva T.A., Minsk, 2022, pp. 377–382. (Russian)
Shlyahtun A.H., Sutsko I.P., Buksha E.V., et al. Protektornoe dejstvie predshestvennikov biosinteza nikotinamidadenindinukleotida na funkczional`noe sostoyanie mitokhondrii pecheni pri alkogol`noj intoksikaczii [Protective effect of precursors of nicotinamide adenine dinucleotide biosynthesis on liver mitochondrial functions in alcohol intoxication]. Biokhimiya i molekulyarnaya biologiya [Biochemistry and Molecular Biology]. 2023, vol. 2, iss. 3, pp. 14–21. (Russian)
Liu Y., Cheng C., Gao H., et al. Restoring energy metabolism by NAD+ supplement prevents alcohol-induced liver injury and boosts liver regeneration. Food Sci. Nutr., 2024, vol. 12, iss. 7, pp. 5100–5110. DOI: 10.1002/fsn3.4159
Yang X., Zheng E., Lin Y., et al. β-Nicotinamide mononucleotide alleviates alcohol-induced liver injury in a mouse model through activation of NAD+/SIRT1 signaling pathways. Hereditas, 2025, vol. 162, iss. 1, article 161. DOI: 10.1186/s41065-025-00529-x
Liu R., Visscher J. A novel preparation of nicotinamide mononucleotide. Nucleosides Nucleotides., 1994, vol. 13, iss. 5, pp. 1215–1216. DOI: 10.1080/15257779408011891.
Close B., Banister K., Baumans V., et al. Recommendations for euthanasia of experimental animals: Part 1. DGXI of the European Commission. Lab Anim., 1996, vol. 30, iss. 4, pp. 293–316. DOI: 10.1258/002367796780739871
Duncombe W. G. The colorimetric micro-determination of non-esterified fatty acids in plasma. Clinica Chim. Acta, 1964, vol. 9, pp. 122–125. DOI: 10.1016/0009-8981(64)90004-x
Folch J., Lees M., Sloane S.G.H. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 1957, vol. 226, iss. 1, pp. 497–509.
de la Maza M. P., Hirsch S., Nieto S., et al. Fatty acid composition of liver total lipids in alcoholic patients with and without liver damage. Alcohol Clin. Exp. Res., 1996, vol. 20, iss. 8, pp. 1418–1422. DOI: 10.1111/j.1530-0277.1996.tb01143.x
Buko V.U., Sushko L.I. Role of microsomal ethanol-oxidizing system in regulation of linoleoyl-CoA desaturase activity after long-term ethanol administration. Alcohol Alcohol., 1988, vol. 23, iss. 1, pp. 69–71.
