Dynamics of gelatinolytic activity of Pleurotus ostreatus deep culture on a nutrient media of different composition
Keywords:
mycelial culture, nutrient media, protein concentration, intracellular and extracellular gelatinolytic proteinasesAbstract
Growth dynamics, level of intracellular and extracellular protein, gelatinolytic activity of intracellular and extracellular proteinases during cultivation of Pleurotus ostreatus mycelium for 14 days on potato “decoction + sucrose” medium and Chapek-Dox medium with water-soluble starch were studied. It was found that when mycelium growing on Chapek-Doks medium after 12 and 14 days, the protein concentration in mycelium exceeded that when using a potato-sucrose medium by 86 and 69%, respectively. The protein concentration in the culture fluid on both nutrient media did not change significantly over the entire culture period. When the fungus grew on both nutrient media, the gelatinolytic activity of the mycelium appeared at pH 5.8, 7.6, 9.2 and 10.6. However, the maximum activity at these pH values in the case of a potato-sucrose nutrient medium appeared at different times. On the Chapek-Doks medium, maximal proteolytic activity at all pH corresponded to 12 days after the beginning of culture growth. The specific activity of proteinases per 1 mg of mycelium protein when the fungus growing on a potato-sucrose medium exceeded that on Chapek-Doks medium by 2.28-2.61 times, with the exception of the activity at pH 10.2. However, the specific activity of extracellular proteinases per 1 mg of protein with growth of the fungus on Chapek-Dox medium was more than seven times (P < 0.05) higher than the activity of the culture fluid of the fungus when it was cultured on potato-sucrose medium.
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