FeCl3, CuSO4, CoCl2, ZnSO4 and NiSO4 effect on a gelatinolytic activity of proteinases of Pleurotus ostreatus mycelial culture
Keywords:
gelatinolytic intracellular proteases, fungal mycelium culture, effect of FeCl3, CuSO4, CoCl2, ZnSO4, NiSO4Abstract
The effect of FeCl3, CuSO4, CoCl2, ZnSO4 and NiSO4 at a concentration of 10–8–10–2 M on the gelatinolytic activity of intracellular proteinases from in 14 days mycelial culture of Pleurotus ostreatus was studied. At pH 5.8, all salts inhibited activity within 30%. Only zinc sulfate in several concentrations contributed to a weak increase in proteinase activity: up to 16%.
The gelatinolytic activity at pH 7.6 is characterized by the activating effect of copper sulfate in the entire range of concentrations, reaching 25–67%. The gelatinolytic activity upon the addition of cobalt chloride was suppressed more strongly than in an acidic medium at a CoCl2 concentration of 10–6 M, by 53%. At the same time, at two maximum concentrations of this effector, gelatinolysis increased by 25 and 31%.
The maximum activator effect of nickel sulfate manifested itself at a concentration of 10–2 M and reached 137%, while at concentrations of 10–4 and 10–5 M, proteolysis was suppressed by 26 and 22%.
At pH 9.2, CuSO4 had a clear activating effect (up to 157% relative to control) in almost the entire concentration range. The effect of other salts did not exceed 24%.
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