Effect of orthophosphate level in nutrient medium on Pinnularia diatome culture growth and intracellular protein content
Keywords:
diatoms, biomass, protein, orthophosphateAbstract
Exclusion of orthophosphate from the Tamiya nutrient medium caused inhibition of Pinnularia diatome culture growth, accumulation of intracellular protein and cell death on the 17th day.
In the range of orthophosphate concentrations of 2.2•10–8–2.2•10–5 M, the concentration dependence of the biomass dynamics was complex. Even a decrease in the concentration of orthophosphate in the nutrient medium was not always accompanied by inhibition of culture growth, and in some cases its level was not inferior to the original Tamiya medium (control). Perhaps this reflects the mechanisms of cell adaptation, and not the toxic effect of the phosphorus salt.
Despite the linear dynamics of the increase in intracellular protein content, at all concentrations of orthophosphate in the nutrient medium, the protein level was significantly lower than the control variant. Perhaps this reflects a non-optimal ratio of the phosphorus level with other components of the nutrient medium, primarily the nitrogen source.
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