Влияние уровня ортофосфата в питательной среде на рост культуры Pinnularia diatome и содержание внутриклеточного белка
Ключевые слова:
диатомовые водоросли, биомасса, белок, ортофосфатАннотация
Исключение из питательной среды Тамийя ортофосфата вызвало угнетение роста культуры Pinnularia diatome, накопления внутриклеточного белка и гибели клеток на17-е сутки. В диапазоне концентраций ортофосфата2,2•10–8–2,2•10–5 М концентрационная зависимость динамики биомассы носила сложный характер. Даже уменьшение концентрации ортофосфата в питательной среде не всегда сопровождалось подавлением роста культуры, и в ряде моментов ее уровень не уступал оригинальной среде Тамийя (контроль). Возможно, это отражает механизмы адаптации клеток, а не токсический эффект соли фосфора.
Несмотря на носящую линейный характер динамику нарастания содержания внутриклеточного белка, при всех концентрациях ортофосфата в питательной среде уровень белка был значительно ниже контрольного варианта. Возможно, это отражает неоптимальное соотношение уровня фосфора с другими компонентами питательной среды, прежде всего – источника азота.
Библиографические ссылки
Algae–bacteria interactions: еvolution, ecology and emerging applications / R. Ramanan [et al.] // Biotechnology аdvances. – 2016. – Vol. 34, iss. 1. – P. 14–29. https://doi.org/10.1016/j.biotechadv.2015.12.003
A time-calibrated multi-gene phylogeny of the diatom genus Pinnularia / C. Souffreau [et al.] // Molecular рhylogenetics and еvolution. – 2011. – Vol. 61, iss. 3. – P. 866–879. https://doi.org/10.1016/j.ympev.2011.08.031
Applications of diatoms as potential microalgae in nanobiotechnology / А.А. Jamali [et al.] // Bioimpacts. – 2012. – Vol. 2, iss. 2. – Р. 83–89. doi: 10.5681/bi.2012.012
Diatoms biotechnology: various industrial applications for a greener tomorrow / N. Sharma [et al.] // Frontiers in marine science. – 2021. – Vol. 8. doi: 10.3389/fmars.2021.636613
Hamed, А. A brief and general overview on diatoms and their applications: review / A. Hamed // Egyptian journal of phycology. – 2023. – Vol. 24, iss. 1. – Р. 1–53. 10.21608/EGYJS.2023.218427.1019
Diatoms in wastewater treatment: potentials, applications, and values of biomass / Т.Т. Rahman [et al.] //. Critical reviews in environmental science and technology. – 2024. – Vol. 54, iss. 7. – P. 557–580. https://doi.org/10.1080/10643389.2023.2259278
Seckbach, J. Diatoms: fundamentals and applications / J. Seckbach, R. Gordon. – Scrivener Publishing, Wiley Global Headquarters, USA, 2019. – 663 р. doi:10.1002/9781119370741
Optimization of the growth and marennine production by the diatom Haslea ostrearia in photobioreactor / R.N. Xuan [et al.] // Algal research. – 2021. – Vol. 55, iss. 2. – Р. 1–17. doi:10.1016/j.algal.2021.102251
Enhancement of biomass production of diatom Nitzschia sp. S5 through optimisation of growth medium composition and fed-batch cultivation / M. Grubišić [et al.] // Marine Drugs. – 2024. – Vol. 22 (1), iss. 46. – P. 4–22 https://doi.org/10.3390/md22010046
Duncan, K. Metallothioneins and related chelators. metal ions in life sciences, vol. 5. / K. Duncan ; edited by A. Sigel, H. Sigel, R.K.O. Sigel // Angewandte chemie international edition. – 2009. – Vol. 48, iss. 43. – Р. – 7966–7967. https://doi.org/10.1002/anie.200903833
Deepmala, К. Effect of phosphate on growth of diatoms/ К. Deepmala // Indian journal of scientific research. – 2011. – Vol. 1, iss. 2. – Р. 103–106.
Excessive phosphorus caused inhibition and cell damage during heterotrophic growth of Chlorella regularis / Q. Li [et al.] // Bioresource technology. – 2018. – Vol. 268. – Р. 266–270. doi: 10.1016/j.biortech.2018.07.148
Enzymatic colorimetric quantifi cation of orthophosphate / В. Li [et al.] // The japanese society of applied glycoscience. – 2011. – Vol. 58. – Р. 125–127. DOI: 10.5458/jag.jag.JAG-2011_002
Akinnawo, S.О. Eutrophication: causes, consequences, physical, chemical and biological techniques for mitigation strategies / S.О. Akinnawo // Environmental challenges. – 2023. – Vol. 12. – Р. 1–18. https://doi.org/10.1016/j.envc.2023.100733.
The effects of limiting phosphate on photosynthesis and growth of Lotus japonicas / R. Thuynsma [et al.] // South african journal of botany. – 2016. – Vol. 104. – P. 244–248. https://doi.org/10.1016/j.sajb.2016.03.001.
Effect of orthophosphate and bioavailability of dissolved organic phosphorous compounds to typically harmful cyanobacterium Microcystis aeruginosa / J. Li [et al.] // Marine pollution bulletin. – 2015. – Vol. 92, iss. 1–2. – Р. 52–58. DOI: 10.1016/j.marpolbul.2015.01.001.
Mitryasova, О. Prognosis models of nitrates and orthophosphates content in surface waters / О. Mitryasova, А. Shybanov, Е. Dzhumelia // Environmental safety and natural resources. – 2022. – Vol. 44, iss. 4. – Р. 30–43. doi:10.32347/2411-4049.2022.4.30-43
Zelnik, I. Diversity and structure of the tychoplankton diatom community in the limnocrene spring Zelenci (Slovenia) in relation to environmental factors / I. Zelnik, T. Balanč, M.J. Toman // Water. – 2018. – Vol. 10, iss. 4, art. 361. – Р. 1 – 12. https://doi.org/10.3390/w10040361
Badamasi, H. Impacts of phosphates on water quality and aquatic life / H. Badamasi [et al.] // Chemistry research journal. – 2019. – Vol. 4, iss.3. – Р. 124–133.
Красовский, П.Г. Выделение культуры диатомовой водоросли и ее рост на различных питательных средах / П.Г. Красовский // Научный потенциал молодежи – будущему Беларуси : материалы XVI международной молодежной научно–практической конференции, Пинск, 15 апреля 2022 г. – Пинск : ПолесГУ, 2022. – Ч. 2. – С. 234–237.
Ильючик, И.А. Методические рекомендации по изучению биохимических свойств одноклеточных зеленых водорослей (на примере Chlorella vulgaris) / И.А. Ильючик, В.Н. Никандров. – Пинск : ПолесГУ, 2020. – 29 с.
References
Ramanan R., Kim B.-H., Cho D.-H., Oh H.-M., Kim H.-S. Algae–bacteria interactions: еvolution, ecology and emerging applications. Journal of Biotechnology Аdvances, 2016, Vol. 34(1), рр. 14–29. https://doi.org/10.1016/j.biotechadv.2015.12.003
Souffreau C., Verbruggen H., Wolfe A.P., Vanormelingen P., Siver P.A., Cox E.J., Mann D.G., Vijver B.V., Sabbe K., Vyverman W. A time-calibrated multi-gene phylogeny of the diatom genus Pinnularia. Journal of Molecular Phylogenetics and Evolution. 2011, Vol. 61(3), рр. 866–879. https://doi.org/10.1016/j.ympev.2011.08.031
Jamali A.A., Akbari F., Ghorakhlu M.M., de la Guardia M, Yari Khosroushahi A. Applications of diatoms as potential microalgae in nanobiotechnology. Journal of Bioimpacts, 2012, Vol. 2(2), рр. 83 – 89. DOI: 10.5681/bi.2012.012
Sharma N, Simon D.P., Diaz-Garza A.M., Fantino E., Messaabi A., Meddeb-Mouelhi F., Germain H., Desgagné-Penix I. Diatoms biotechnology: Various Industrial Applications for a Greener Tomorrow, Frontiers in Marine Science, 2021, Vol. 8. doi: 10.3389/fmars.2021.636613
Hamed А. A brief and general overview on diatoms and their applications: review. Egyptian Journal of Phycology, 2023, Vol. 24(1), рр. 1–53. 10.21608/EGYJS.2023.218427.1019
Rahman T.T., Jiang T., Zhang C., Rao Y., Sims R. C., Hou, L. Diatoms in wastewater treatment: Potentials, applications, and values of biomass. Critical Reviews in Environmental Science and Technology, 2023, Vol. 54(7), рр. 557–580. https://doi.org/10.1080/10643389.2023.2259278
Seckbach J., Gordon R. Diatoms: fundamentals and applications. Scrivener Publishing, Wiley Global Headquarters, USA, 2019, 663 р. DOI:10.1002/9781119370741
Xuan R.N., Mouget J.L., Turpin V., Jaouen P., Pruvost J. Optimization of the growth and marennine production by the diatom Haslea ostrearia in photobioreactor. Journal of Algal Research, 2021, Vol. 55(2), рр. 1–17. DOI:10.1016/j.algal.2021.102251
Grubišić, M.; Šantek, B.; Kuzmić, M.; Čož-Rakovac, R.; Ivančić Šantek, M. Enhancement of Biomass Production of Diatom Nitzschia sp. S5 through Optimisation of Growth Medium Composition and Fed-Batch Cultivation. Journal of Marine Drugs 2024, 22, 46. https://doi.org/10.3390/md22010046
Duncan K. Metallothioneins and Related Chelators. Metal Ions in Life Sciences, Vol. 5. Edited by Sigel А., Sigel H., Sigel R.K.O. Angewandte Chemie International Edition, 2009, Vol. 48(43), рр. 7966–7967. https://doi.org/10.1002/anie.200903833
Deepmala К. Effect of Phosphate on Growth of Diatoms. Indian Journal of Scientific Research, 2011, Vol. 1(2), рр. 103–106.
Li Q, Fu L, Wang Y, Zhou D, Rittmann B.E. Excessive phosphorus caused inhibition and cell damage during heterotrophic growth of Chlorella regularis. Journal of Bioresour Technology, 2018, Vol. 268, рр. 266–270. doi: 10.1016/j.biortech.2018.07.148.
Bingxue Li, Takanori N., Hiroyuki N., Mamoru N., Motomitsu K. Enzymatic colorimetric quantifi cation of orthophosphate. Journal of The Japanese Society of Applied Glycoscience, 2011, Vol. 58, рр. 125–127. DOI: 10.5458/jag.jag.JAG-2011_002
Akinnawo S.О Eutrophication: Causes, consequences, physical, chemical and biological techniques for mitigation strategies. Journal of Environmental Challenges, 2023, Vol. 12, рр. 1–18. https://doi.org/10.1016/j.envc.2023.100733.
Thuynsma R., Kleinert А., Kossmann J., Valentine A.J., Hills P.N. The effects of limiting phosphate on photosynthesis and growth of Lotus japonica. South African Journal of Botany, 2016, Vol. 104, рр. 244–248. https://doi.org/10.1016/j.sajb.2016.03.001.
Li J., Wang Z., Cao X., Wang Z., Zheng Z. Effect of orthophosphate and bioavailability of dissolved organic phosphorous compounds to typically harmful cyanobacterium Microcystis aeruginosa. Journal of Marine pollution bulletin, 2015, Vol. 92(1–2), рр. 52–58. DOI: 10.1016/j.marpolbul.2015.01.001.
Mitryasova O., Shybanova A., Dzhumelia E. Prognosis models of nitrates and orthophosphates content in surface waters. Journal of Environmental safety and natural resources, 2022, Vol. 44(4), рр. 30–43. DOI:10.32347/2411-4049.2022.4.30-43
Zelnik I.; Balanč T.; Toman M.J. Diversity and Structure of the Tychoplankton Diatom Community in the Limnocrene Spring Zelenci (Slovenia) in Relation to Environmental Factors. Journal of Water 2018, Vol. 10(4):361, рр. 1 – 12. https://doi.org/10.3390/w10040361
Badamasi H., Yaro, M.N., Ibrahim A., Aliyu Bashir I. Impacts of Phosphates on Water Quality and Aquatic Life. Chemistry research journal, 2019, Vol. 4(3), рр. 124–133.
Krasovsky P.G. Vydelenie kul'tury diatomovoj vodorosli i ee rost na razlichnyh pitatel'nyh sredah [Isolation of diatom culture and its growth on various nutrient media]. Nauchnyj potencial molodezhi – budushchemu Belarusi : materialy XVI mezhdunarodnoj molodezhnoj nauchno–prakticheskoj konferencii, Pinsk, 15 aprelya 2022 g. Pinsk : PolesGU, 2022, pp. 234–237. (In Russian)
Ilyuchyk I.A., Nikandrov, V.N. Metodicheskie rekomendatsii po izucheniiu biokhimicheskikh svoistv odnokletochnykh zelenykh vodoroslei (na primere Chlorella vulgaris) [Methodological recommendations for studying the biochemical properties of unicellular green algae (using the example of Chlorella vulgaris)]. Pinsk, PolesGU, 2020, 29 p. (In Russian)
